Wine authenticity is a major concern worldwide, with around 5% of the wines being mislabeled and sold in secondary markets. Thus, methods suitable to combat some fraudulent practices in the wine sector are imperative.
DNA-based methodologies are a reliable mean of tracking wine varietal composition. However, DNA recovery from wine samples is very difficult and yields very fragmented DNA molecules, requiring the use DNA markers that are both highly informative and small sized.
Single Nucleotide Polymorphism (SNP) markers have demonstrated to be highly recommended in forensic samples, and have been widely applied to grapevine varietal identification, joining the two requests necessary for the development of a DNA-based authenticity system.
High-resolution melting (HRM) represents a method that enables the genotyping of SNPs in a large number of samples and is highly reproducible between labs.
This HRM assay demonstrated to be highly sensitive, rapid and efficient when applied to wine samples and was transferable to commercial wine samples, revealing to be suitable for wine authenticity purposes.
Report presented at the SIVE OENOPPIA Awards 2019. The paper reproduced in this video-seminar was presented at the 12th edition of Enoforum (Vicenza, Italy, May 21-23, 2019).