Dimopoulou Maria1, Goulioti Elli2, Troianou Vicky3, Toumpeki Chrisavgi3, Gosselin Yves4, Dorignac Etienne4, Kontoudakis Nikolaos2, Kotseridis Yorgos2
1 Department of Wine, Vine and Beverage Sciences, School of Food Science, University of West Attica, Greece
2 Agricultural University of Athens, Laboratory of Enology, Iera Odos 75, 11855, Athens Greece
3 Innovino Research&Development, Meg. Alexandrou 21, Pallini 15351, Greece
4 Fermentis 137 rue Gabriel Péri, 59703 Marcq en Baroeul, France

Email contact: mdimopoulou[@]uniwa.gr

Enhancing the sensory profile of wine by the use of different microorganism has been always a challenge in winemaking. The aim of our work was to evaluate the impact of different fermentation schemes by using mixed and pure cultures of  different Saccharomyces species to Sauvignon blanc wine chemical composition and sensory profile.

Sauvignon blanc must has been inoculated with mixed and pure cultures of S. pastorianus and S. cerevisiae. For the mixed fermentation schemes, one strain of S. pastorianus has been inoculated under different frequencies (99%, 95% , 90%, 80% and 70%) with two strains of S. cerevisiae. Totally 13  fermentations trials, 3 monocultures and 10 mixed cultures, were realised in triplicate. The fermentation kinetics has been controlled by density measurement and classic oenological  analysis (residual sugars, total acidity, volatile acidity, malic acid degradation, glycerol production etc) were performed based on OIV protocols.The population dynamics was conducted by the specific interdelta PCR reaction of the Saccharomyces species in the beginning and in the end of the fermentation process. Volatile aromatic compounds such as esters, superior alcohols and thiols were evaluated by GC/MS analysis. Sensory assesement was carried out  for all wines by trained panel.

All fermentation trials lead to dryness and the fermentation lasted from 9 days  to 13 days. The population dynamics analysis revealed that the S. cerevisiae strain was the most predominant in the end of the fermentation process  in any inoculation ratio tested. The wines fermented with S. pastorianus, either in pure or mixed cultures, were characterised by significant lower acetic acid production and greater malic acid degradation compared to the wines fermented with S. cerevisiae strains. The aromatic profile of the produced wines was highly affected from the inoculation ratio while the effect of the S. cerevisiae used strain was less important.

Our study based on different fermentation frequencies  of  mixed cultures of S. pastorianus and S. cerevisiae strains, revealed the impact of the inoculation ratio on the 30 tested volatiles compounds,  correlated to Sauvignon blanc aromatic profile. The species of S. pastorianus starts to become an interesting candidate for co-inoculation with S. cerevisae strains, able to boost varietal aromas intensity.

  • Dimopoulou M., Troianou V., Toumpeki C., Gosselin Y., Dorignac E., Kotseridis Y. (2020). Effect of strains from different Saccharomyces species used in different inoculation schemes on chemical composition and sensory characteristics of Sauvignon blanc wine. OENO One, 54, 4.
  • Albertin, W., Masneuf-Pomarède, I., Galeote, V., & Legras, J.-L., (2019). New insights into wine yeast diversities, in Romano, P., Ciani, M., and Fleet, G. H. (Eds.), Yeasts in the Production of Wine, New York, NY, Springer, pp. 117–163.
  • Sadoudi, M., Tourdot-Maréchal, R., Rousseaux, S., Steyer, D., Gallardo-Chacón, J.-J., Ballester, J., Vichi, S., Guérin-Schneider, R., Caixach, J., & Alexandre, H., (2012). Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts, Food Microbiology, 32, 2, 243–253.
Related sheets: