Wine authenticity based on High Resolution Melting

Paula Martins-Lopes, University of Tras-os-Montes and Alto Douro, BioISI, Portugal

Wine authenticity based on High Resolution Melting

Wine authenticity is a major concern worldwide, with around 5% of the wines being mislabeled and sold in secondary markets. Thus, methods suitable to combat some fraudulent practices in the wine sector are imperative.

DNA-based methodologies are a reliable mean of tracking wine varietal composition. However, DNA recovery from wine samples is very difficult and yields very fragmented DNA molecules, requiring the use DNA markers that are both highly informative and small sized.

Single Nucleotide Polymorphism (SNP) markers have demonstrated to be highly recommended in forensic samples, and have been widely applied to grapevine varietal identification, joining the two requests necessary for the development of a DNA-based authenticity system.

High-resolution melting (HRM) represents a method that enables the genotyping of SNPs in a large number of samples and is highly reproducible between labs.

This HRM assay demonstrated to be highly sensitive, rapid and efficient when applied to wine samples and was transferable to commercial wine samples, revealing to be suitable for wine authenticity purposes.

Report presented at the SIVE OENOPPIA Awards 2019. The paper reproduced in this video-seminar was presented at the 12th edition of Enoforum (Vicenza, Italy, May 21-23, 2019).

Annual Subscription to Infowine: The subscription – to the price of € 60,00 (VAT included) – gives unlimited access for one year to all the documents published on the website: Click here

Published on 11/02/2020
Item available in italiano
Premium Contents Area
  • VIDEO SEMINARIO (Paula MARTINS-LOPES; streaming 15 min)
Price:27 €(Tax included)
Related sheets
© All Right Reserved
ISSN 1826-1590 VAT: IT01286830334
powered by Infonet Srl Piacenza
- A +
ExecTime : 4,736328