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Effects of different antioxidant strategies on the phenolic evolution during the course of a white winemaking process

Antonio Castro Marin1, Federico Baris1, Fabio Chinnici1

1 Department of food science, University of Bologna, Italy

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This work aimed to evaluate the evolution of phenolic compounds during white winemaking process up to bottling and 12 months storage, together with the influence of different antioxidant strategies (e.g. fining on lees and addition of sulfur dioxide, ascorbic acid, glutathione, and chitosan) on the overall kinetics.

To this purpose, a mass spectrometric approach has been adopted by using HPLC-MS/MS, in order to get new insights in the understanding of wine oxidation processes.

Sulphonated compounds related to oxidation were identified (e.g. S-sulfonated glutathione, and tryptophol and indole-3-lactic sulfonates) and their production was revealed to occur after alcoholic fermentation or fining on lees and to increase after 10 months of storage. On the other hand, treatments with chitosan during winemaking seemed linked to the hydrolysis of hydroxycinnamates, releasing their corresponding hydroxycinnamic acids. Surprisingly, when present during storage in bottle a particular behavior of chitosan was observed, where this biopolymer avoided the phenomenon of hydrolysis and showed higher inhibition against phenolic products of oxidation such as hydroxycaffeic acid dimers. Furthermore, the addition of ascorbic acid to chitosan-treated wines before bottling, reduced the generation of oxidations products and raised the production of GRP derived phenols, correlated to a better protection against oxygen. The absorption properties of chitosan with regard to phenolics were also proposed to have some consequences on the evolution of wine browning.

Based on these results, the knowledge about polyphenols fate may represent a useful approach to manage the antioxidant strategies during winemaking processes.  



Arapitsas, P., Guella, G., & Mattivi, F. (2018). The impact of SO2 on wine flavanols and indoles in relation to wine style and age. Scientific Reports, 8(1), 1–13.

Bradshaw, Marc P., Barril, C., Clark, A. C., Prenzler, P. D., & Scollary, G. R. (2011). Ascorbic Acid: A Review of its Chemistry and Reactivity in Relation to a Wine Environment. Critical Reviews in Food Science and Nutrition, 51(6), 479–498.

Castro, A., Culcasi, M., Cassien, M., Stocker, P., Thétiot-laurent, S., Robillard, B., … Pietri, S. (2019). Chitosan as an antioxidant alternative to sulphites in oenology?: EPR investigation of inhibitory mechanisms. Food Chemistry, 285(September 2018), 67–76.

Chinnici, F., Natali, N., & Riponi, C. (2014). Efficacy of Chitosan Inhibiting the Oxidation of (+)-Catechin in White Wine Model Solutions. Journal of Agricultural and Food Chemistry, (62), 9868–9875.

Published on 06/18/2018
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