Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-Up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification The optimal method consisted of passing wine, diluted with an aqueous Solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 mu g L-1 according to EU legislation was achieved with both formals. A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the inembiane-based assay. The proposed rapid gel-based test was compared with a standard immunoaffinity column – high-performance liquid chromatography – fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine samples (We recommend that you consult the full text of this article).