A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers. (We advise you to read the entire article. Original title : Optimization of interdelta analysis for Saccharomyces cerevisiae strain characterization)
Abstract The management and disposal of large amounts of grape..
Ayuso, S.1 Fernández-Pérez, R.2 Moreta, C.1 Tenorio, Carmen and 1Ruiz-Larrea,..
The demand for a sustainable economy and resource conservation is..
The seasonal character of harvests limits winemaking considerably, especially oenological..
