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Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine

Rusanova, TY; Beloglazova, NV; Goryacheva, IY; Lobeau, M; Van Peteghem, C; De Saeger, S. 2009. ANALYTICA CHIMICA ACTA 653 (1): 97-102

Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-Up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification The optimal method consisted of passing wine, diluted with an aqueous Solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 mu g L-1 according to EU legislation was achieved with both formals. A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the inembiane-based assay. The proposed rapid gel-based test was compared with a standard immunoaffinity column - high-performance liquid chromatography - fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine samples (We recommend that you consult the full text of this article).
Published on 10/05/2010
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