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OPTIMIZATION OF INTERDELTA ANALYSIS FOR SACCHAROMYCES CEREVISIAE STRAIN CHARACTERIZATION

Legras, Jean-Luc; Karst, François, FEMS Microbiology Letters, 2003, Vol. 221, n°2, p249-255

A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers. (We advise you to read the entire article. Original title : Optimization of interdelta analysis for Saccharomyces cerevisiae strain characterization)

Published on 21/08/2003
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