• » Technical Articles
  • » Cell viability and proteins release during ultrasound-assisted yeast lysis of light lees in model wine

Cell viability and proteins release during ultrasound-assisted yeast lysis of light lees in model wine

Juan Francisco García Martín, Lucien Guillemet, Chaohui Feng, Da-Wen Sun; Food Chemistry, Volume 141, Issue 2, 15 November 2013, Pages 934–939

Light lees that spent more than one year in barrels were used for ultrasound-assisted yeast lysis (22 W/L, 18 °C) in a model wine. For comparison, a classical yeast autolysis at mild temperature (25 °C) was performed.

The effect of ultrasound on lees was evaluated by analysing the release of proteins and polysaccharides to the model wine, and the viability of the yeasts contained in the lees.

Under conditions tested, ultrasound-assisted yeast lysis increased the concentrations of proteins and polysaccharides in the model wine due to the release of these compounds from yeasts.

Unlike the classical autolysis, ultrasound led to a high cell disruption, and after 20 h of ultrasonic treatment, viable cells were hardly found.

Furthermore, the final cell concentration for the ultrasound-assisted yeast lysis was much lower than that for the classical autolysis. The inactivation rate constant of ultrasound-assisted yeast lysis was 2.54 × 10−5 s−1.

Finally, the morphological changes in cells were examined by scanning electron microscopy to verify the effect of ultrasound on yeast cells.

(We recommend that you consult the full text of this article)


Published on 10/02/2013
Related sheets
© All Right Reserved
ISSN 1826-1590 VAT: IT01286830334
powered by Infonet Srl Piacenza
Privacy Policy
This website and its related third-party services make use of cookies necessary for the purposes described in the cookie policy. If you want to learn more about cookies or how to disable them (either totally or partially), please see the cookie policy. By closing this banner, scrolling through this page, clicking on a link or continuing navigation in any other way, you consent to the use of cookies.
More informationOK

- A +
ExecTime : 1,757813