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Roles of proteins, polysaccharides, and phenolics in haze formation in white wine via reconstitution experiments.

Gazzola, D.; Sluyter, S. C. van; Curioni, A.; Waters, E. J.; Marangon, M.; 2012, Journal of Agricultural and Food Chemistry 60 (42) 10666–10673

Residual proteins in finished wines can aggregate to form haze. To obtain insights into the mechanism of protein haze formation, a reconstitution approach was used to study the heat-induced aggregation behaviour of purified wine proteins.


 A chitinase, 4 thaumatin-like protein (TLP) isoforms, phenolics, and polysaccharides were isolated from a Chardonnay wine. The same wine was stripped of these compounds and used as a base to reconstitute each of the proteins alone or in combination with the isolated phenolics and/or polysaccharides. 


After a heating and cooling cycle (70°C for 1 h and 25°C for 15 h), the size and concn. of the aggregates formed were measured by scanning ion occlusion sensing (SIOS), a technique to detect and quantify nanoparticles. 


The chitinase was the protein most prone to aggregate and the 1 that formed the largest particles; phenolics and polysaccharides did not have a significant impact on its aggregation behaviour. 


TLP isoforms varied in susceptibility to haze formation and in interactions with polysaccharides and phenolics. 


The work establishes SIOS as a useful method for studying wine haze


(We recommend that you consult the full text of this article)


Published on 11/12/2013
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